| 1. | In addition , the heparinase is stimulated in the presence of ca2 + and mn2 + , but is inhibited by cu2 + and fe3 + . neither pmsf nor edta significantly affected enzyme activity . by infrared spectrum and proton resonance spectrum analysis , we found smaller polysaccharides , unsaturated bond and reducing sugar , which are specific products by heparinase
在产物分析中发现，该酶作用于肝素后，还原糖数量明显增加，通过红外色谱及质子谱的分析发现，产物中出现了含不饱和双键的还原寡糖，这是肝素酶作用于肝素后的特异性产物，从而可以进一步证明该菌确实产生肝素酶。 |
